Administration of Garlic Essential Oil Restored the Altered Enzymatic and Non-enzymatic Parameters and Pulmonary Histoarchitecture in Mice Subjected to Lead Nitrate

Jump To References Section

Authors

  • Surabhi Gupta
     Department of Bioscience and Biotechnology, Banasthali Vidyapith, Tonk - 304022, Rajasthan ,IN
  • Veena Sharma
     Department of Bioscience and Biotechnology, Banasthali Vidyapith, Tonk - 304022, Rajasthan ,IN

DOI:

https://doi.org/10.18311/jnr/2024/35969

Keywords:

Allium sativum, Lead Nitrate, Lung

Abstract

The focus of the current research work was to unfold the therapeutic potential of Garlic Essential Oil (GEO) in altered oxidative stress, biochemical parameters and histoarchitecture of pulmonary tissue of mice intoxicated with the inorganic salt of Lead. Thirty six (36) mice were used in the experiment, and they were divided into 6 groups, with 6 mice in each group. The experimental groups were as: control/untreated, Lead Nitrate (LN), LN + low dose of GEO, LN + high dose of GEO, LN with standard drug (silymarin) and LN with vehicle olive oil. The total duration of the experimental study was of 30 days. The outcome of the study showed downstream levels of SOD, CAT, GPx, GSH and TPC and upstream levels of LPO, total level of cholesterol, LDH, ADH and GGT. Toxicant exposure also de-structured the pulmonary tissue and on the other side both low and high doses of GEO, standard compound silymarin and vehicle olive oil improved the altered enzymatic and non-enzymatic parameters and re-structured the distorted pulmonary tissue to a greater extent. Thus, it is concluded that GEO plays a vital role in imparting protection to lung tissue from lead poisoning.

Downloads

Download data is not yet available.

Metrics

Metrics Loading ...

Downloads

Published

2024-08-31

How to Cite

Gupta, S., & Sharma, V. (2024). Administration of Garlic Essential Oil Restored the Altered Enzymatic and Non-enzymatic Parameters and Pulmonary Histoarchitecture in Mice Subjected to Lead Nitrate. Journal of Natural Remedies, 24(8), 1695–1707. https://doi.org/10.18311/jnr/2024/35969

Issue

Volume 24, Issue 8, August 2024

Section

Research Articles

Categories

License

Copyright (c) 2024 Surabhi Gupta, Veena Sharma (Author)

Creative Commons License

This work is licensed under a Creative Commons Attribution 4.0 International License.

Crossref
Scopus
Google Scholar
Europe PMC
Received 2023-12-24
Accepted 2024-07-15
Published 2024-08-31

 

References

Sokol RZ, Berman N. The effect of age of exposure on leadinduced testicular toxicity. Toxicology. 1991; 69(3):26978. https://doi.org/10.1016/0300-483X(91)90186-5 PMid:1949051

Wilson EA, Demmig‐Adams B. Antioxidant, antiinflammatory, and antimicrobial properties of garlic and onions. NFS. 2007; 37(3):178-83. https://doi.org/10.1108/00346650710749071

Imai J, Ide N, Nagae S, Moriguchi T, Matsuura H, Itakura Y. Antioxidant and radical scavenging effects of aged garlic extract and its constituents. Planta Medica. 1994; 60(05):41720. https://doi.org/10.1055/s-2006-959522 PMid:7997468

O’Gara EA, Hill DJ, Maslin DJ. Activities of garlic oil, garlic powder, and their diallyl constituents against Helicobacter pylori. Applied and Environmental Biology. 2000; 66(5):2269-73. https://doi.org/10.1128/AEM.66.5.22692273.2000 PMid:10788416 PMCid: PMC101489

Tsao SM, Yin MC. In-vitro antimicrobial activity of four diallyl sulphides occurring naturally in garlic and Chinese leek oils. Journal of Medicinal Microbiology. 2001; 50(7):646-9. https:// doi.org/10.1099/0022-1317-50-7-646 PMid:11444776

Corzo-Martínez M, Corzo N, Villamiel M. Biological properties of onions and garlic. Trends in Food Science and Technology. 2007; 18(12):609-25. https://doi.org/10.1016/j.tifs.2007.07.011

Fuller NJ, Pegg RB, Affolter J, Berle D. Variation in growth and development, and essential oil yield between two Ocimum species (O. tenuiflorum and O. gratissimum) grown in Georgia. Hort Sci. 2018; 53(9):1275-82. https:// doi.org/10.21273/HORTSCI13156-18

Singh R, Sharma V, Sharma S. Histological study of a hepatoprotective aspect of indigo and its isolated isothiocyanate compound against N-Nitrosopyrrolidine intoxicated mice.

Sharma V, Sharma A, Kansal L. The effect of oral administration of Allium sativum extracts on lead nitrate induced toxicity in male mice. Foog and Chemical Toxicology. 2010; 48(3):928-36. https://doi.org/10.1016/j.fct.2010.01.002 PMid:20060875

Zeng T, Zhang CL, Zhu ZP, Yu LH, Zhao XL, Xie KQ. Diallyl trisulfide (DATS) effectively attenuated oxidative stressmediated liver injury and hepatic mitochondrial dysfunction in acute ethanol-exposed mice. Toxicology. 2008; 252(1-3):8691. https://doi.org/10.1016/j.tox.2008.07.062 PMid:18755235

Kang YM, Shin SC, Jin YW, Kim HS. Changes in body and organ weights, haematological parameters, and frequency of micronuclei in the peripheral blood erythrocytes of ICR mice exposed to low-dose-rate $gamma $-radiation. Journal of Radiation Protection and Research. 2009; 34(3):102-6.

Sasaki YF, Nishidate E, Izumiyama F, Matsusaka N, Tsuda S. Simple detection of chemical mutagens by the alkaline single-cell gel electrophoresis (Comet) assay in multiple mouse organs (liver, lung, spleen, kidney, and bone marrow). Mutations Research/Genetic Toxicology and Environmental Mutagenesis. 1997; 391(3):215-31. https:// doi.org/10.1016/S1383-5718(97)00073-9 PMid:9268047

Dhindsa RS, Plumb-Dhindsa PA, Thorpe TA. Leaf senescence: correlated with increased levels of membrane permeability and lipid peroxidation, and decreased levels of superoxide dismutase and catalase. Journal of Experimental Biology. 1981; 32(1):93-101. https://doi.org/10.1093/jxb/32.1.93

Lewden O, Garcher C, Morales C, Javouhey A, Rochette L, Bron AM. Changes of catalase activity after ischemia-reperfusion in rat retina. Ophthalmic research. 1996; 28(6):331-5. https:// doi.org/10.1159/000267924 PMid:9032790

Flohé L, Günzler W. Assays of glutathione peroxidase. In methods in enzymology. 1984; 105:114-20. Academic Press. https://doi.org/10.1016/S0076-6879(84)05015-1 PMid:6727659

Ellman GL. Tissue sulfhydryl groups. Archives of biochemistry and biophysics. 1959; 82(1):70-7. https://doi.org/10.1016/0003-9861(59)90090-6 PMid:13650640

Plastunov B, Zub S. Lipid peroxidation processes and antioxidant defence under lead intoxication and iodinedeficient in the experiment. Uniy Marie Curie Sklodwska Lublin Polonia. 2008; 21:215-7. https://doi.org/10.2478/ v10080-008-0118-6

Bradford MM. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Analytical biochemistry. 1976; 72(1-2):248-54. https://doi.org/10.1006/abio.1976.9999 PMid:942051

Gerson T. The determination of cholesterol and coprostanol in faecal lipids. Biochemical Journal. 1960; 77(3):446. https://doi.org/10.1042/bj0770446 PMid:13704757 PMCid:PMC1205055

Bergmeyer HU, editor. Methods of enzymatic analysis. Elsevier; 2012.

Vallee BL, Hoch FL. Zinc is a component of yeast alcohol dehydrogenase. Processing of the National Academy of Science. 1955; 41(6):327-38. https://doi.org/10.1073/ pnas.41.6.327 PMid:16589675 PMCid:PMC528091

Hanigan MH, Lykissa ED, Townsend DM, Ou CN, Brarrios R, Lieberman MW. Gamma-glutamyl transpeptidase-deficient mice are resistant to the nephrotoxic effects of cisplatin. The American journal of pathology. 2001; 159(5):1889-94. https:// doi.org/10.1016/S0002-9440(10)63035-0 PMid:11696449

Cardoso AD, e Silva CP, Dos Anjos FD, Quesnot N, da Maia Valenca H, Cattani-Cavalieri I, Brito-Gitirana L, Valenca SS, Lanzetti M. Diallyl disulfide prevents cigarette smokeinduced emphysema in mice. Pulmonary Pharmacology and Therapeutics. 2021; 69:102053. https://doi.org/10.1016/j.pupt.2021.102053 PMid:34214692

Ahamed M, Siddiqui MK. Low-level lead exposure and oxidative stress: current opinions. Clinica chimica acta. 2007; 383(1-2):57-64. https://doi.org/10.1016/j.cca.2007.04.024 PMid:17573057

Omurtag GZ, Güranlioğlu FD, Şehirli Ö, Arbak S, Uslu B, Gedik N, Şener G. Protective effect of aqueous garlic extract against naphthalene‐induced oxidative stress in mice. Journal of Pharmacy and Pharmacology. 2005; 57(5):623-30. https://doi.org/10.1211/0022357055939 PMid:15901351

Bechara EJ. Lead poisoning and oxidative stress. Free radical Biology and Medicine. 2004; 36:S22-res. https://doi.org/10.1016/j.freeradbiomed.2004.02.047

Masella R, Di-Benedetto R, Varì R, Filesi C, Giovannini C. Novel mechanisms of natural antioxidant compounds in biological systems: involvement of glutathione and glutathionerelated enzymes. Journal of Nutritional Biochemistry. 2005; 16(10):577-86. https://doi.org/10.1016/j.jnutbio.2005.05.013 PMid:16111877

Hussein AA. Mechanism of action of the Egyptian garlic "Allium sativum" on excitable tissues. Pak J Bio Sci. 2003; 6(8):782-8. https://doi.org/10.3923/pjbs.2003.782.788

Kamata T, Jin H, Giblett S, Patel B, Patel F, Foster C, Pritchard C. The cholesterol‐binding protein NPC 2 restrains the recruitment of stromal macrophage‐lineage cells to earlystage lung tumours. EMBO Molecular Medicine. 2015; 7(9):1119-37. https://doi.org/10.15252/emmm.201404838 PMid:26183450 PMCid:PMC4568947

Sodimu O, Joseph PK, Augusti KT. Certain biochemical effects of garlic oil on rats maintained on a high fat-high cholesterol diet. Experientia. 1984; 40:78-80. https://doi.org/10.1007/BF01959111 PMid:6692895

Bhatwalkar SB, Mondal R, Krishna SB, Adam JK, Govender P, Anupam R. Antibacterial properties of organosulfur compounds of garlic (Allium sativum). Frontiers in Microbiology. 2021; 12:1869. https://doi.org/10.3389/fmicb.2021.613077 PMid:34394014 PMCid:PMC8362743

Corti A, Belcastro E, Dominici S, Maellaro E, Pompella A. The dark side of Gamma-Glutamyltransferase (GGT): Pathogenic effects of an ‘antioxidant ‘ enzyme. Free Radical Biology and Medicine. 2020; 160:807-19. https://doi.org/10.1016/j.freeradbiomed.2020.09.005 PMid:32916278