Biocontrol potential of endophytic Trichoderma sp. against the pathogenic fungus, Alternaria alternata that causes leaf spot in tomato plants
DOI:
https://doi.org/10.18311/jbc/2023/33458Keywords:
Alternaria alternata, phenols, tomato plant, Trichoderma harzianum, Trichoderma koningiiAbstract
This study was aimed at evaluating the two isolates of the bio-resistant fungi, Trichoderma harzianum and T. koningii in management of the fungus that causes Alternaria alternata leaf spot disease in tomato plants by inducing systemic resistance in the plant. It was demonstrated that two isolates of the pathogenic fungus, A. alternata could infect tomato seeds on water agar media. Isolate No. 1 was the most successful, with an infection rate of 63%, while isolate No. 2 achieved an infection rate of 56%. When employed in concentrations of (10, 20, or 30%) with PDA culture medium, bio-resistant fungus filtrate demonstrated suppression of the pathogenic fungus A. alternata, with the enhanced levels of inhibition with increasing concentration used. Oxacycloheptadec-8-en-2-one, compound 9,12-Octadecadienoic acid, methyl ester, and many more chemical compounds with the ability to inhibit fungi were discovered via the use of the GC-MS equipment to analyze the fungal infiltrates produced by T. harzianum and T. koningii. The bio-resistant fungi significantly lessened the severity of the infection caused by the pathogenic fungus, A. alternata, reaching a reduction of 33.81% during treatment with T. harzianum as opposed to injury of 56.855% in pathogen alone. The tomato leaves that were treated with T. konhngii produced the maximum phenolic content (0.56 mg/g).
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Copyright (c) 2023 ABDULNABI A. A. MATROOD, KHALIL-BERDI FOTOUHIFAT
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
Accepted 2023-08-18
Published 2023-10-10
References
Abdel-Kader, M. M., El-Mougy, N. S., Aly, M. D. E, Lashin, S. M., and El-Mohamady, R. S. 2012. Soil drench with fungicides alternatives against root rot incidence of some vegetables under greenhouse conditions. Int J Agric For, 2(2): 61-69. https://doi.org/10.5923/j.ijaf.20120202.10 DOI: https://doi.org/10.5923/j.ijaf.20120202.10
Agrios, G. N. 1997. Plant Pathology (4th Ed). Academic Press Inc. New York, 635.
Chen, Y. J., Meng, Q., Zeng, L., and Tong, H. R. 2018. Phylogenetic and morohological characteristics of Alternaria alternata causing leaf spot disease Oncamellia sinensis in China. Australas Plant Pathol, 47(3): 335-342. https://doi.org/10.1007/s13313-018-0561-0 DOI: https://doi.org/10.1007/s13313-018-0561-0
Deguzman, F. S., Gloer, J. B., Wicklow, D. T., and Dowd, P. F. 1992. New diketopiperazine metabolites from The sclerotia of Aspergillus ochraceus. J Nat Prod, 55(7): 931-939. https://doi.org/10.1021/np50085a013 DOI: https://doi.org/10.1021/np50085a013
Douglas, S. K., Jhon, J. R., and Schad, E. J. 1984. Alternaria toxin and their important in food. J Food Prot, 47: 886- 901. https://doi.org/10.4315/0362-028X-47.11.886 DOI: https://doi.org/10.4315/0362-028X-47.11.886
Fontenelle, A. D. B., Guzzo, S. D., Lucon, C. M. M., and Harakava, R. 2011. Growth promotion and induction of resistance in tomato plant against Xanthomonas euvesicatoria and Alternaria solani by Trichoderma sp. Crop Prot, 30(11): 1492-1500. https://doi.org/10.1016/j.cropro.2011.07.019 DOI: https://doi.org/10.1016/j.cropro.2011.07.019
Jabnoun-Khiareddine, H., Abdallah, R., El-Mohamedy, R., Abdel-Kareem, F., Gueddes C-Chahed, M., Hajlaoui, A., and Daami-Remadi, M. 2016. Comparative efficacy of potassium salts against soil-borne and air-borne fungi and their ability to suppress tomato wilt and fruit rots. J. Microbiol Biotechnol, 8(2): 45-55.
Matrood, A.A.A. 2018. Biocontrol of the cladosporic spot in the eggplant plant caused by the fungus Cladosporium cladosporioides. Arab J Plant Prot, 36(3): 192-198. DOI: https://doi.org/10.22268/AJPP-036.3.192198
Matrood, A. A. A., and Rhouma, A. 2021a. Efficacy of foliar fungicides on controlling early blight disease of eggplant, under laboratory and greenhouse conditions. Nov Res Microbiol J, 5: 1283-1293. https://doi. org/10.21608/nrmj.2021.178310 DOI: https://doi.org/10.21608/nrmj.2021.178310
Matrood, A. A. A., Rhouma, A., and Okon, G. O. 2021b. Evaluation of the biological control agent’s efficiency against the causal agent of early blight of Solanum melongena. Arab J Plant Prot, 39(3): 204-209. DOI: https://doi.org/10.22268/AJPP-039.3.204209
Matrood, A. A. A, Rhouma, A., and Mohammed, T. F. 2022. Control of Fusarium wilt disease of cucumber using rhizospheric antagonistic fungi. Arab J Plant Prot, 40(1): 62-69. https://doi.org/10.22268/AJPP-040.1.062069 DOI: https://doi.org/10.22268/AJPP-040.1.062069
Meena, R. K., Patni, K., and Arora, D. K. 2008. Study on phenolics and their oxidative enzyme in Capsicum annuum L. infected with gemini virus. As J Exp Sci, 22(3): 307-310.
Melfi, M. T., Nardiello, D., Cicco, N., Candido, V., and Centonze, D. 2018. Simultaneous determination of water-and fat-soluble vitamins, lycopene and betacarotene in tomato samples and pharmaceutical formulations: Double injection single run by reversephase liquid chromatography with UV detection. J Food Compos Anal, 70: 9-17. https://doi.org/10.1016/j.jfca.2018.04.002 DOI: https://doi.org/10.1016/j.jfca.2018.04.002
Poly, S., and Srikanta, D. 2013. Assessment of losses due to early blight (Alternaria solani Ell. and Mart.) and influence of weather factors on disease development in tomato. J Agrometeorol, 15(1): 82-85. https://doi.org/10.54386/jam.v15i1.1449 DOI: https://doi.org/10.54386/jam.v15i1.1449
Zain. M. E. (2009). Effect of olive oil on secondary metabolite and fatty acid profiles of Penicillium expansum, Aspergillus flavus, A. parasiticus and A. ochraceus. Aust J Basic Appl Sci, 3(4): 4274-4280.